Host-induced modification-restriction and the utilization of 5-bromouracil by thymineless mutants of Escherichia coli.
نویسندگان
چکیده
An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K.
منابع مشابه
Utilization of 5-bromouracil by thymineless bacteria.
Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be ea...
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Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that transitions and possibly transversions are produced. This system th...
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Lederberg, Seymour (Brown University, Providence, R.I.). Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J. Bacteriol. 91:1029-1036. 1966.-The locus for the host specific restriction and modification of deoxyribonucleic acid in Escherichia coli has been mapped by matings between mutants for these characters in strains K-12, C600, and B. Lin...
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عنوان ژورنال:
- Journal of bacteriology
دوره 104 1 شماره
صفحات -
تاریخ انتشار 1970